LOCUS       sequence-83673-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     pZac2.1gfaABC1Dlck-GCaMP6f
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-lck-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(169..177,178..192)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      199..208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..276
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     489..510
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(616..637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgtggctgca gctcaaaccc tgaagatgac tggatggaga acattgacgt gtgtgagaac
       61 tgccattatc ccccgccgaa gcttcgaatg gatccagcca ccatgggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatctcgc caccatggtc gactcatccc gtcgtaagtg gaataagaca
      241 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
      301 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
      361 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      421 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
      481 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      541 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
      601 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      661 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      721 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta c
//
