LOCUS       sequence-83675-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express EGFP-Kir4.1 fusion protein in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Tong X, Ao Y, Faas GC, Nwaobi SE, Xu J, Haustein MD, Anderson MA, 
            Mody I, Olsen ML, Sofroniew MV, Khakh BS
  TITLE     Astrocyte Kir4.1 ion channel deficits contribute to neuronal 
            dysfunction in Huntington's disease model mice.
  JOURNAL   Nat Neurosci. 2014 May;17(5):694-703. doi: 10.1038/nn.3691. Epub 
            2014 Mar 30.
  PUBMED    24686787
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3691"; 
            journalName: "Nat Neurosci"; date: "2014-05"; volume: "17"; issue: 
            "5"; pages: "694-703"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-EGFP-Kir4.1
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(24..45)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(285..304)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     632..653
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
       61 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      121 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      181 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      241 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      301 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      361 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      421 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      481 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      541 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      601 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      661 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
      721 cttcgaattc tgttgccaag gtctattaca gccagacgac gca
//
