LOCUS       sequence-83676-e        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt3 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt3
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7..48
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gccaccatgg ggagtagcaa gagcaagcct aaggacccca gccagcgcag cgatggagag
       61 gaagagatgg atgcctctac aacccatcat aaaagaaaga caatgaatga ttttgactat
      121 ttgaaactac taggtaaagg cacttttggg aaagttattt tggttcgaga gaaggcaagt
      181 ggaaaatact atgctatgaa gattctgaag aaagaagtca ttattgcaaa ggatgaagtg
      241 gcacacactc taactgaaag cagagtatta aagaacacta gacatccctt tttaacatcc
      301 ttgaaatatt ccttccagac aaaagaccgt ttgtgttttg tgatggaata tgttaatggg
      361 ggcgagctgt ttttccattt gtcgagagag cgggtgttct ctgaggaccg cacacgtttc
      421 tatggtgcag aaattgtctc tgccttggac tatctacatt ccggaaagat tgtgtaccgt
      481 gatctcaagt tggagaatct aatgctggac aaagatggcc acataaaaat tacagatttt
      541 ggactttgca aagaagggat cacagatgca gccaccatga agacattctg tggcactcca
      601 gaatatctgg caccagaggt gttagaagat aatgactatg gccgagcagt agactggtgg
      661 ggcctagggg ttgtcatgta tgaaatgatg tgtgggaggt tacctttcta caaccaggac
      721 catgagaaac tttttgaatt aatattaatg gaagacatta aatttcctcg aacactctct
      781 tcagatgcaa aatcattgct ttcagggctc ttgataaagg atccaaataa acgccttggt
      841 ggaggaccag atgatgcaaa agaaattatg agacacagtt tcttctctgg agtaaactgg
      901 caagatgtat atgataaaaa gcttgtacct ccttttaaac ctcaagtaac atctgagaca
      961 gatactagat attttgatga agaatttaca gctcagacta ttacaataac accacctgaa
     1021 aaatatgatg aggatggtat ggactgcatg gacaatgaga ggcggccgca tttccctcaa
     1081 ttttcctact ctgcaagtgg acgagaataa
//
