LOCUS       sequence-83677-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Haustein MD, Kracun S, Lu XH, Shih T, Jackson-Weaver O, Tong X, Xu 
            J, Yang XW, O'Dell TJ, Marvin JS, Ellisman MH, Bushong EA, Looger 
            LL, Khakh BS
  TITLE     Conditions and constraints for astrocyte calcium signaling in the 
            hippocampal mossy fiber pathway.
  JOURNAL   Neuron. 2014 Apr 16;82(2):413-29. doi: 10.1016/j.neuron.2014.02.041.
  PUBMED    24742463
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.02"; journalName: "Neuron"; date: 
            "2014-04-16- 16"; volume: "82"; issue: "2"; pages: "413-29"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-cyto-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..40
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(44..52,53..67)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..157
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     370..391
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(497..518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
       61 cgataaggat ctcgctagcg ccaccatggt cgactcatca cgtcgtaagt ggaataagac
      121 aggtcacgca gtcagagcta taggtcggct gagctcactc gagaacgtct atatcaaggc
      181 cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg
      241 cggcgtgcag ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      301 gctgcccgac aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa
      361 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      421 cgagctgtac aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac
      481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      541 gtccggcgag ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      721 cgaaggctac atccaggagc gcaccatctt cttcaaggac gacggcaact
//
