LOCUS       sequence-83697-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L-(+)-arabinose-inducible expression of link-CGFP negative control 
            for in vivo split GFP assembly assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, Hamilton AD, 
            Regan L
  TITLE     Detecting protein-protein interactions with a green fluorescent 
            protein fragment reassembly trap: scope and mechanism.
  JOURNAL   J Am Chem Soc. 2005 Jan 12;127(1):146-57.
  PUBMED    15631464
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2005-01-12"; volume: "127(1)"; pages: "146-57"
COMMENT     Sequence Label: pMRBAD-link-CGFP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     312..332
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(426..444)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      440..487
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
       61 tttgtttaac tttaagaagg agatataccc atggctaatg catgcaatgg gacgtcgggt
      121 ggaagcggta agaatggaat caaagtgaac ttcaagaccc gccacaacat tgaagatgga
      181 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      241 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      301 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      361 gaactgtaca actaaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      421 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      481 ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga
      541 tggtgaccac catttgcccc atatcaccgt cggtactgat cccgtcgtca ataaaccgaa
      601 ccgctacacc ctgagcatca aactctttta tcagttggat catgtcggcg gtgtcgcggc
      661 caagacggtc gagcttcttc accagaatga catcaccttc ctccaccttc atcctcagca
      721 aatccagccc ttcccgatct gttgaactgc cggatgcctt gtcggtaaag atgcggttag
      781 cttttacccc tgcatctttg agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc
      841 atac
//
