LOCUS       sequence-83700-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to 
            amber codons, for use in phosphoprotein synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Rinehart Lab Phosphoprotein synthesis components
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PCRT7 tetR pLtetO MBP-MEK1 XX Amp
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..32
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             33..53
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 acaataacaa caacctcggg atcgagggaa gggaaaatct gtattttcaa ggtcatatgc
       61 cgaagaagaa accgaccccg atccagctga acccggctcc ggacggttct gctgttaacg
      121 gcacctcttc tgctgaaacc aacctggaag ctctgcaaaa gaaactggaa gaactggaac
      181 tggacgaaca gcagcgtaaa cgtctggaag cgttcctgac ccagaaacag aaagttggtg
      241 aactgaaaga cgacgacttc gaaaaaatct ctgaactggg tgctggtaac ggtggtgttg
      301 ttttcaaagt ttctcacaaa ccgtccggtc tggttatggc tcgtaaactg atccacctgg
      361 aaatcaaacc ggctatccgt aaccagatca tccgtgaact gcaagttctg cacgaatgca
      421 actctccgta catcgttggt ttctacggtg ctttctactc tgacggtgaa atctctatct
      481 gcatggaaca catggacggt ggttctctgg accaggttct gaaaaaagct ggtcgtatcc
      541 cggaacagat cctgggtaaa gtttctatcg ctgttatcaa aggtctgacc tacctgcgtg
      601 aaaaacacaa aatcatgcac cgtgacgtta aaccgtctaa catcctggtt aactctcgtg
      661 gtgaaatcaa actgtgcgac ttcggtgttt ctggtcagct gatcgactag atggctaact
      721 agttcgttgg cacccgttct tacatgtctc cggaacgtct gcaaggcacc cactactctg
      781 ttcagtctga catctggtct atgggtctgt ctctggttga aatggctgtt ggtcgttacc
      841 cgatcccgcc gcc
//
