LOCUS       sequence-83701-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to 
            amber codons, for use in phosphoprotein synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Rinehart Lab Phosphoprotein synthesis components
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PCRT7 tetR pLtetO MBP-MEK1 XX Amp
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..74)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc aagctagctt gtcgacggag ctcgaattcg gatccggcgc gccttagtga
       61 tggtggtgat gatgcgcagc tgcggctgcc acaccagcag cgtgggtcgg ggtagacggc
      121 tggttcagac cgatggtaga gcacagccaa ccagcgaagt caacttcttc agcgtcagaa
      181 cgtttgatga aagcgtgaac catcagctgt ttcaggtcag cacgttcagc cgggtttttg
      241 atcaggcatt tgttaacgaa gtcctggaac tccagagaga aaacaccaga cggcagtttc
      301 ggaggcggtt cgttaacgat gtagtccagc agttcgaaga tagccatcgg cggacgagag
      361 tccataccgt aagaagacag cggacgaccc ggagtacgcg gacgcggcgg ggtttcagca
      421 gcgtcacctt caacctggca accgaacatc agttccagtt ctttagcgtc cggcggcggg
      481 atcgggtaac gaccaacagc catttcaacc agagacagac ccatagacca gatgtcagac
      541 tgaacagagt agtgggtgcc ttgcagacgt tccggagaca tgtaagaacg ggtgccaacg
      601 aactagttag ccatctagtc gatcagctga ccagaaacac cgaagtcgca cagtttgatt
      661 tcaccacgag agttaaccag gatgttagac ggtttaacgt cacggtgcat gattttgtgt
      721 ttttcacgca ggtaggtcag acctttgata acagcgatag aaactttacc caggatctgt
      781 tccgggatac gaccagcttt tttcagaacc tggtccagag aaccaccgtc catgtgttcc
      841 atgcagatag agatttcacc gtcagagtag aaagcaccgt agaaaccaac gatgtacgga
      901 gagttgcatt
//
