LOCUS       sequence-83702-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4BD-tagged Hepatitis B splicing protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Chen JY, Chen WN, Jiao BY, Lin WS, Wu YL, Liu LL, Lin X
  TITLE     Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects
            of benzo [alpha] pyrene by interacting with microsomal epoxide 
            hydrolase and enhancing its hydrolysis activity.
  JOURNAL   BMC Cancer. 2014 Apr 23;14:282. doi: 10.1186/1471-2407-14-282.
  PUBMED    24758376
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Cancer."; date: "2014-04-23"; volume: "14:282. doi"; pages: " 
            10.1186/1471-2407-14-282"
COMMENT     Sequence Label: pBIND-HBSP
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(417..857)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(888..906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(949..968)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agcattaacc ctcactaaag ggaagcggcc ttagttattc aggtacctgc ggccgctcta
       61 gactagtaaa ctgagccagg agaaacggac tgaggcccac tcccatagga atcttgcgaa
      121 agcccaggat gatgggatgg gaatacaagt gcagtttccg tccgaaggtt ttgtacagca
      181 acaagaggga aacatagagg ttccttgagc aggaatcgtg caggtcttgc atggccccgt
      241 gctggtggtt gatgctcatt gagattcccg agattgagat cttctgcgac gcggcgattg
      301 agaccttcgt ctgcgaggcg agggagttct tcttctaggg gacctgcctc gtcgtctaac
      361 aacagtagtt tccggaagtg ttgataagat aggggacgga tccccgggaa ttccggcgat
      421 acagtcaact gtctttgacc tttgttacta ctctcttccg atgatgatgt cgcacttatt
      481 ctatgctgtc tcaatgttag aggcatatca gtctccactg aagccaatct atctgtgacg
      541 gcatctttat tcacattatc ttgtacaaat aatcctgtta acaatgcttt tatatcctgt
      601 aaagaatcca ttttcaaaat catgtcaagg tcttctcgag gaaaaatcag tagaaatagc
      661 tgttccagtc tttctagcct tgattccact tctgtcagat gtgccctagt cagcggagac
      721 cttttggttt tgggagagta gcgacactcc cagttgttct tcagacactt ggcgcacttc
      781 ggtttttctt tggagcactt gagcttttta agtcggcaaa tatcgcatgc ttgttcgata
      841 gaagacagta gcttcatctt tcaggaggct tgcttcaagc tggctagcct atagtgagtc
      901 gtattaagta ctctagcctt aagagctgta attgaactgg gagtggacac ctgtggagag
      961 aaaggcaaag tggatgtcag taagaccaat aggtgcctat cagaaacgca agagtcttct
     1021 ctgtctcgac aagcccagtt tctattggtc tccttaaacc tgtcttgtaa ccttga
//
