LOCUS       sequence-83704-e       11729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea 
            agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby 
            produces epsilon-carotene in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the 
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
  PUBMED    15659105
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005-02"; volume: "41(3)"; pages: "478-92"
COMMENT     Sequence Label: pAC-EPSILON
FEATURES             Location/Qualifiers
     source          1..11729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7164..7183)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7437..7459
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7451..7468
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7452..7468
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7475..7494
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7475..7493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7518..7534
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7519..7535
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9373..9389)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9373..9389)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9426..9444)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9465..9481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9465..9481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9478..9500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9489..9505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9513..9519,9520..9537,9538..9543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9558..9579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(join(11289..11729,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggaatg cgggctgggc tatttcaccc taccactggc tattcgctgc
     3841 cgctggcggt ggcccttgcc gacgcgattg ccgacagccc gcggctgggc agcgttccgc
     3901 tctatcagct cacccggcag tttgccgaac gccactggcg caggcaggga ttcttccgcc
     3961 tgctgaaccg gatgcttttc ctggccgggc gcgaggagaa ccgctggcgg gtgatgcagc
     4021 gcttttatgg gctgccggag cccaccgtag agcgctttta cgccggtcgg ctctctctct
     4081 ttgataaggc ccgcattttg acgggcaagc caccggttcc gctgggcgaa gcctggcggg
     4141 cggcgctgaa ccattttcct gacagacgag ataaaggatg aaaaaaaccg ttgtgattgg
     4201 cgcaggcttt ggtggcctgg cgctggcgat tcgcctgcag gcggcaggga tcccaaccgt
     4261 actgctggag cagcgggaca agcccggcgg tcgggcctac gtctggcatg accagggctt
     4321 tacctttgac gccgggccga cggtgatcac cgatcctacc gcgcttgagg cgctgttcac
     4381 cctggccggc aggcgcatgg aggattacgt caggctgctg ccggtaaaac ccttctaccg
     4441 actctgctgg gagtccggga agaccctcga ctatgctaac gacagcgccg agcttgaggc
     4501 gcagattacc cagttcaacc cccgcgacgt cgagggctac cggcgctttc tggcttactc
     4561 ccaggcggta ttccaggagg gatatttgcg cctcggcagc gtgccgttcc tctcttttcg
     4621 cgacatgctg cgcgccgggc cgcagctgct taagctccag gcgtggcaga gcgtctacca
     4681 gtcggtttcg cgctttattg aggatgagca tctgcggcag gccttctcgt tccactccct
     4741 gctggtaggc ggcaacccct tcaccacctc gtccatctac accctgatcc acgcccttga
     4801 gcgggagtgg ggggtctggt tccctgaggg cggcaccggg gcgctggtga acggcatggt
     4861 gaagctgttt accgatctgg gcggggagat cgaactcaac gcccgggtcg aagagctggt
     4921 ggtggccgat aaccgcgtaa gccaggtccg gctggcggat ggtcggatct ttgacaccga
     4981 cgccgtagcc tcgaacgctg acgtggtgaa cacctataaa aagctgctcg gccaccatcc
     5041 ggtggggcag aagcgggcgg cagcgctgga gcgcaagagc atgagcaact cgctgtttgt
     5101 gctctacttc ggcctgaacc agcctcattc ccagctggcg caccatacca tctgttttgg
     5161 tccccgctac cgggagctga tcgacgagat ctttaccggc agcgcgctgg cggatgactt
     5221 ctcgctctac ctgcactcgc cctgcgtgac cgatccctcg ctcgcgcctc ccggctgcgc
     5281 cagcttctac gtgctggccc cggtgccgca tcttggcaac gcgccgctgg actgggcgca
     5341 ggaggggccg aagctgcgcg accgcatctt tgactacctt gaagagcgct atatgcccgg
     5401 cctgcgtagc cagctggtga cccagcggat ctttaccccg gcagacttcc acgacacgct
     5461 ggatgcgcat ctgggatcgg ccttctccat cgagccgctg ctgacccaaa gcgcctggtt
     5521 ccgcccgcac aaccgcgaca gcgacattgc caacctctac ctggtgggcg caggtactca
     5581 ccctggggcg ggcattcctg gcgtagtggc ctcggcgaaa gccaccgcca gcctgatgat
     5641 tgaggatctg caatgagcca accgccgctg cttgaccacg ccacgcagac catggccaac
     5701 ggctcgaaaa gttttgccac cgctgcgaag ctgttcgacc cggccacccg ccgtagcgtg
     5761 ctgatgctct acacctggtg ccgccactgc gatgacgtca ttgacgacca gacccacggc
     5821 ttcgccagcg aggccgcggc ggaggaggag gccacccagc gcctggcccg gctgcgcacg
     5881 ctgaccctgg cggcgtttga aggggccgag atgcaggatc cggccttcgc tgcctttcag
     5941 gaggtggcgc tgacccacgg tattacgccc cgcatggcgc tcgatcacct cgacggcttt
     6001 gcgatggacg tggctcagac ccgctatgtc acctttgagg atacgctgcg ctactgctat
     6061 cacgtggcgg gcgtggtggg tctgatgatg gccagggtga tgggcgtgcg ggatgagcgg
     6121 gtgctggatc gcgcctgcga tctggggctg gccttccagc tgacgaatat cgcccgggat
     6181 attattgacg atgcggctat tgaccgctgc tatctgcccg ccgagtggct gcaggatgcc
     6241 gggctgaccc cggagaacta tgccgcgcgg gagaatcggg ccgcgctggc gcgggtggcg
     6301 gagcggctta ttgatgccgc agagccgtac tacatctcct cccaggccgg gctacacgat
     6361 ctgccgccgc gctgcgcctg ggcgatcgcc accgcccgca gcgtctaccg ggagatcggt
     6421 attaaggtaa aagcggcggg aggcagcgcc tgggatcgcc gccagcacac cagcaaaggt
     6481 gaaaaaattg ccatgctgat ggcggcaccg gggcaggtta ttcgggcgaa gacgacgagg
     6541 gtgacgccgc gtccggccgg tctttggcag cgtcccgttt aggcgggcgg ccatgacgtt
     6601 cacgcaggat cgcctgtagg tcggcaggct tgcgggcgta aataaaaccg aaggagacgc
     6661 agccctcccg gccgcgcacc gcgtggtgca ggcggtgggc gacgtagagc cgcttcaggt
     6721 agccccggcg cgggatcccg cctcgccgtg ctgtccggtc tcaacctgat ccgccagaat
     6781 cgagccaacg ggatcggcca gcacgaattc ggtatgcgga gagtgtttgg caaaccatgc
     6841 ctgcaagcca cccagcgtgc cgccggagcc aacgcctact accacggcat caaccctgcc
     6901 tgccagctgg tcgaacagct ccggtgcggt ggtggtggcg tgcgccagcg ggttggccgg
     6961 gttagagaac tgatcaatat agtaagcacc cggcgtctcc tctgccaggc ggcgggcgta
     7021 gtcctgatag tactccgggt ggccctttgt cacgtcggag cgggtcaggc gcacatcaac
     7081 acccagcgca cgcaggtggt agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     7141 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     7201 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     7261 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     7321 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     7381 actggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     7441 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     7501 gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcgcggc
     7561 cgcttaaaaa acatataatt aacgagtatg ttgttgtaag gatacaaaag aaataaataa
     7621 ggcttatata tatgcaagat atgggtatta tttatataat ctaaatctat atagtgagat
     7681 atgcttttac catagttgcc cctgtcggat cagaaagtag atgtctaacc agttccattc
     7741 tcaagctgtg aggtgctatc acaaacatat aaagcgcaaa tattatcaaa tccgttgaag
     7801 ataaagaaga ccccaaaaat ccccaccaca tccatttggg caaacgaaag aaagtacgga
     7861 aaaatgtacg tgttccctct agatcnatta gcacgatgtg tgatagtccg aatagaaaga
     7921 aggctcgctg tcttttcctt tcaagtggcc acaatgtttc ccatgcttgt tttgaaatgt
     7981 tagtgtattt tccaagagaa atcatctctt tagattgatc ttgtcttaaa atcttagcaa
     8041 tgactgctgc ataattagga gcttctgaca aagatcgaac aactgaatac cctgtggcag
     8101 ggtgcaccat actagctgca gcaccaaatg cgagattctt ttgttctgta ttaggtaacg
     8161 atccacctac ggggatatac gaccattcct cttcgtacgt tcttgttatt cggataccca
     8221 ttgcctttaa tcgtgacatg agtttggact ttagaagatt gaaaggcatg gcttctcttg
     8281 aagctaaaca agtttcctcg aagaatattt ttgttggaga catggccatg acatagagga
     8341 aagtcggata ttttgcttct aaagattccg gtttatgttt tgagaagtct ctataatcca
     8401 tgaacaccat tagatctgga tcataggggt tgttttcaac ctcaacctct ataccataag
     8461 ctgtttggac acaaacacgg ggacccccaa gttcatactc cagaaatttc cctgaagctg
     8521 cccctgatgc aacagtagca agcctgcatg gaatggtgat attgccttca cattcaatga
     8581 gactatagcc atttggagct tcagtgattc tttctacttt ggagcttaga tatgaaacac
     8641 ctgattccac acaccttctt aacaactctt catgaagtaa atcacgatga actctgccat
     8701 atgcacgacc tatgcggatg ggatcagcat catcaaggta tacaagagta tctttccaag
     8761 aatgttcaat gcatccttca agtccaagac ctataaattc atcctgccaa acaccataat
     8821 tgtttgtaaa aggaagatca gggccaatga gtccaacgtt caaccctagt ttggctgact
     8881 ctgcagcaag agcaaggcca gcagggccac aaccgattac aaccagatca agtatgcaat
     8941 ttccaattgg tatctgtgct agcttttcgg aaagtttaga ctggctttcc atggacttag
     9001 tccgctgcat ttgaacaaaa aacagctccg atccaccggc cttcacataa tcttcttcgt
     9061 ctgctacgga aatcccttgt ttatccacta cacaacggtc gcttttagcg ctgcacttaa
     9121 tttgacgcaa cgaagacgat gctgataaat tagtaaatct tcgttgtttc agtaacgaaa
     9181 atttgtgcct gatattacag tccgtgaatc tagggcacgt aaaaaccgcc attgttgccg
     9241 tcatgtttcg agctccaaag cactccattg attcttctat cacaaatcaa gttttcagga
     9301 ttacaattaa ttcaattttg aagaactttc acgtcatttg tttcagcggc cgcgaattcc
     9361 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     9421 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     9481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9601 gctttccagt cgggaaacct gtcgtgccag tcgaccgatg cccttgagag ccttcaaccc
     9661 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     9721 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     9781 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     9841 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     9901 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     9961 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
    10021 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
    10081 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
    10141 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
    10201 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
    10261 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
    10321 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
    10381 tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
    10441 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
    10501 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
    10561 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
    10621 tcccctacgt gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg
    10681 atactatgac tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag
    10741 tccgcaccgc tgtccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
    10801 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt
    10861 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg
    10921 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
    10981 acgaagcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
    11041 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
    11101 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
    11161 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
    11221 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
    11281 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
    11341 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
    11401 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
    11461 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
    11521 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
    11581 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
    11641 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
    11701 accagctcac cgtctttcat tgccatacg
//
