LOCUS       sequence-83705-e        4843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an active Arabidopsis thaliana lycopene epsilon-cyclase in 
            E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E.
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Cunningham FX Jr, Pogson B, Sun Z, McDonald KA, DellaPenna D, Gantt 
            E
  TITLE     Functional analysis of the beta and epsilon lycopene cyclase enzymes
            of Arabidopsis reveals a mechanism for control of cyclic carotenoid 
            formation.
  JOURNAL   Plant Cell. 1996 Sep;8(9):1613-26.
  PUBMED    8837512
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell."; date: "1996-09"; volume: "8(9)"; pages: "1613-26"
COMMENT     Sequence Label: y2
FEATURES             Location/Qualifiers
     source          1..4843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2223..2239
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4158..4174)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4159..4175)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4199..4218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4200..4218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4225..4242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4225..4241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4234..4256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4382..4837)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4519..4540)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4731..4750
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtggatcc cccgggctgc aggaattcgc ggccgcgtca ctaaccacaa
     2281 aacaaagact gatttttttc ttttcgtttc catctccatt tcgatcactc cttagattct
     2341 atacatgttt gtttccaact tcctagtaac ccacatacca ttctttgatc gattattcag
     2401 ataaatcaac atatatatac acaaacctaa gagttgataa gtaaatcata ctttgagata
     2461 ggtttttatc atggttgctc cggttggatc agagatgaga tgattgatga gaccttttct
     2521 caaattgttt ggtgaaatga cgaacatgta taaagcaaag agaacgagat ctcctgatgt
     2581 taatgttgat cctagaaacc cttgccacat ccattttgga aggcggaaga aagtacggaa
     2641 gaagcttcta atgccttcgg tatcgaattg aactatgagt gcaagaccaa agagaaagaa
     2701 tgctctctgt cttttccttt ctggtggcca taaagtatcc caagcttgtc ttgaaatatt
     2761 actgttgatc tgtttggtag tctcttctct tagtatctct gcgatgactg atgcatattt
     2821 tggagcttca gacaaagatc tcacaactga atagcctgtt gcgggatgta ccatgctagc
     2881 ggcagcacca aaggcgagat tcttttgttc ggtgtttggc aaggaaccac caactgggat
     2941 ataggaccac tcctcttcgt aagtctttag aattcgaatt ccgagtgtat ctaatcttaa
     3001 catgagcttc gtttttagca aatcaaaggg catgacatct tttgaggcca aacatgtctc
     3061 ctcgaagaag agtcttgact ttgtcatagg catggcgtac agaaacgttg gatactcagc
     3121 ttctaagctc cgaactttct cgttagtata atctctgtaa tccatgaaaa ccatttgatc
     3181 tggatcatat ggactatttt ccacctcaac ctccacgccg tatgcagttt gcacacagac
     3241 tctaggtcca ccaacttcgt attgcaagag ctttcccgaa gctgctccag aagcaacagt
     3301 ggcaagcctg cagggaatga cgttattgtc gtcacaagca acaagtctaa ggccatcaga
     3361 agcttctgtt atgctgtcaa ctttcgagct aaggtacgag acacctgact cgacacacct
     3421 cctcaaaagc tcctcatgga gcaaacgtcg actaactctt ccataagcac ggccaatggt
     3481 aataggcttg tcatcatcca gatacacaat agtctctctc caaacatgct caatacattt
     3541 ttgcagccca agatcattga attcatcttc ccaaacaccg taattgttag taaaaggaag
     3601 atctggacca atgagtccaa cttttaatcc aagcttagct gattctgcag ccaaggctaa
     3661 accagcagga ccacaaccaa taaccactag atccaaagca ccatcaccaa ttgatatagg
     3721 aggcaactta tcaacaagct tagactgttc atccatatct ttgttctgct gcatttgaac
     3781 aaatagaatc tcagaaccac cagctttcac aaaatcttct tcgtcagcga aatcttctct
     3841 caccgctaca caactctcac taccggaact tccgccgccg ctagctctga cactacacaa
     3901 accgaaacga atattcctat agctgtatct cttaaccact ggaaatttcc ttcgacaact
     3961 ccatgacgga aatgttgaaa ccgccattgc tgcgaaattc ctagccccaa cacactccat
     4021 tttcctcctc caaataattt cgaatacagc gagaagactt acaccattgt tatattgttc
     4081 tatcaaggta tagcaagcag aaagaggaat ctaattattt ccttttgtag cggccgcgaa
     4141 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     4201 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4261 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4321 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4381 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4441 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4501 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4561 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4621 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4681 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4741 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4801 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtg
//
