LOCUS       sequence-83709-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  split Venus attached to MCP and PCP respectively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Wu B, Chen J, Singer RH
  TITLE     Background free imaging of single mRNAs in live cells using split 
            fluorescent proteins.
  JOURNAL   Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
  PUBMED    24402470
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-01-9"; volume: "4:3615. doi"; pages: " 
            10.1038/srep03615"
COMMENT     Sequence Label: ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..315)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(106..127)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(712..723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(724..750)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(754..774)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattttgtaa tccagaggtt gattaggatc tatcgcccct gcatgacgta ggtcatcgat
       61 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      121 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      181 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      241 ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
      301 gttcttctgc ttgtcggcgg tggcgaccgg tggatccgca cggcccagcg gcacaaggtt
      361 gacgacaaga tcttcgacct gcgaggtcgc gacgagggac ttggtcaaat cgtacaacga
      421 tttgcgcgag gcctcggtgc tattcgcaac gattgtcacg tcgtgcgacc atacctgagt
      481 gtagcgcact ttcggaagtc cggaatcaac gacgtccgcc tgatccagtt ttaggttgac
      541 gcgatacgcg gtcttggctc cgttttgacg gagcgaagcc gtgaggcgca gccgacccac
      601 cagaggcccg accttctctt cgaagatctg acggtctgcg gtggactgga tctcagtcag
      661 agtgcgagta gcctcgccga ccgaaagaac gatggttttg gaggctagat ggcggccttc
      721 gatggcgtag tcgggcacgt cgtaagggta gccaactttt cttttctttt ttgggcccat
      781 cctgcaggct gtggccatat tatcatcgtg tttttcaa
//
