LOCUS       sequence-83717-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Onishi M, Ko N, Nishihama R, Pringle JR
  TITLE     Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and 
            abscission during yeast cytokinesis.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):311-29. doi: 10.1083/jcb.201302001.
  PUBMED    23878277
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-07-22- 22"; volume: "202"; 
            issue: "2"; pages: "311-29"
COMMENT     Sequence Label: pFA6a-VN155(I153L)-TRP1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(52..54,55..57,58..516)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(97..118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(358..377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     terminator      540..727
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(595..619)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacagat ccatcgccac catggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      181 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      481 tacaactaca acagccacaa cgtctatctc accgcctagg gcgcgccact tctaaataag
      541 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
      601 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
      661 tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct
      721 ctaccggcag atccgctagg gataacaggg taatatagat ctgtacaatc ttgatccgga
      781 gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca
      841 agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc
      901 agct
//
