LOCUS       sequence-83719-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gene deletion with URA3-kanMX6 double marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Onishi M, Ko N, Nishihama R, Pringle JR
  TITLE     Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and 
            abscission during yeast cytokinesis.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):311-29. doi: 10.1083/jcb.201302001.
  PUBMED    23878277
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-07-22- 22"; volume: "202"; 
            issue: "2"; pages: "311-29"
COMMENT     Sequence Label: pFA6a-URA3-KanMX6
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        91..306
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaaggcgc gcctatgcgg catcagagca
       61 gattgtactg agagtgcacc ataccacctt ttcaattcat catttttttt ttattctttt
      121 ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
      181 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
      241 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
      301 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
      361 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
      421 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
      481 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
      541 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
      601 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
      661 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
      721 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
      781 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
      841 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
      901 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
      961 accgtgg
//
