LOCUS       sequence-83721-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(53..240)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     161..185
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(264..974)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(304..324)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     919..947
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtacgctgca ggtcgacgga tctatattac cctgttatcc ctagcggatc tgccggtaga
       61 ggtgtggtca ataagagcga cctcatacta tacctgagaa agcaacctga cctacaggaa
      121 agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta
      181 tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc
      241 ttatttagaa gtggcgcgcc ctatttgtat agttcatcca tgccatgtgt aatcccagca
      301 gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg
      361 gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      421 gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt
      481 ttgaagttag ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag
      541 ttatagttgt attccaattt gtgcccaaga atgtttccat cttctttaaa atcaatacct
      601 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
      661 tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca
      721 ctcttgaaaa agtcatgctg tttcatgtga tctgggtatc ttgaaaagca ttgaacacca
      781 taagtgagag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
      841 aatttaaggg taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat
      901 ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt
      961 tcttctcctt tactgttaat taa
//
