LOCUS       sequence-83724-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(54..241)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     162..186
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(305..325)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgtacgctgc aggtcgacgg atctatatta ccctgttatc cctagcggat ctgccggtag
       61 aggtgtggtc aataagagcg acctcatact atacctgaga aagcaacctg acctacagga
      121 aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt
      181 atacacttat tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg
      241 cttatttaga agtggcgcgc cctatttgta tagttcatcc atgccatgtg taatcccagc
      301 agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag
      361 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
      421 agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat
      481 tttgaagtta gctttgattc cattcttttg tttgtctgcc atgatgtata cattgtgtga
      541 gttatagttg tattccaatt tgtgcccaag aatgtttcca tcttctttaa aatcaatacc
      601 ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt
      661 gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc
      721 actcttgaaa aagtcatgct gtttcatgtg atctgggtat cttgaaaagc attgaacacc
      781 ataagtgaga gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat
      841 aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa
      901 tttgtgccca ttaacatcac catctaattc aa
//
