LOCUS       sequence-83727-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human Connexin 43 resistant to siRNA with Serine to Alanine 
            mutation at 373 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Smyth JW, Zhang SS, Sanchez JM, Lamouille S, Vogan JM, Hesketh GG, 
            Hong T, Tomaselli GF, Shaw RM
  TITLE     A 14-3-3 Mode-1 Binding Motif Initiates Gap Junction Internalization
            during Acute Cardiac Ischemia.
  JOURNAL   Traffic. 2014 Mar 10. doi: 10.1111/tra.12169.
  PUBMED    24612377
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2014 Mar 10. doi: 10.1111/tra.12169."
COMMENT     Sequence Label: pcDNA3.2-GJA1-S373A
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggcttcacc atgggtgact ggagcgcctt aggcaaactc cttgacaagg ttcaagccta
      181 ctcaactgct ggagggaagg tgtggctgtc agtacttttc attttccgaa tcctgctgct
      241 ggggacagcg gttgagtcag cctggggcga cgaacaatcc gcattccgct gtaacactca
      301 gcaacctggt tgtgaaaatg tctgctatga caagtctttc ccaatctctc atgtgcgctt
      361 ctgggtcctg cagatcatat ttgtgtctgt acccacactc ttgtacctgg ctcatgtgtt
      421 ctatgtgatg cgaaaggaag agaaactgaa caagaaagag gaagaactca aggttgccca
      481 aactgatggt gtcaatgtgg acatgcactt gaagcagatt gagataaaga agttcaagta
      541 cggtattgaa gagcatggta aggtgaaaat gcgagggggg ttgctgcgaa cctacatcat
      601 cagtatcctc ttcaagtcta tctttgaggt ggccttcttg ctgatccagt ggtacatcta
      661 tggattcagc ttgagtgctg tttacacttg caaaagagat ccctgcccac atcaggtgga
      721 ctgtttcctc tctcgcccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc
      781 cttggtgtcc ctggccttga atatcattga actcttctat gttttcttca agggcgttaa
      841 ggatcgggtt aagggaaaga gcgaccctta ccatgcgacc agtggtgcgc tgagccctgc
      901 caaagactgt gggtctcaaa aatatgctta tttcaatggc tgctcctcac caaccgctcc
      961 cct
//
