LOCUS       sequence-83731-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chromosomal tagging with stable GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, 
            Pringle JR
  TITLE     Identification of yeast IQGAP (Iqg1p) as an 
            anaphase-promoting-complex substrate and its role in 
            actomyosin-ring-independent cytokinesis.
  JOURNAL   Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17.
  PUBMED    17942599
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-12"; volume: "18(12)"; pages: "5139-53. Epub 
            2007 Oct 17"
COMMENT     Sequence Label: pFA6a-GFP (F64L, S65T, V163A)-His3MX6
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..744
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(61..89)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     684..704
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(823..847)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacagtaaag gagaagaact tttcactgga
       61 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      121 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      181 ggaaaactac ctgttccatg gccaacactt gtcactactc tcacttatgg tgttcaatgc
      241 ttttcaagat acccagatca catgaaacag catgactttt tcaagagtgc catgcccgaa
      301 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      361 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      421 aaagaagatg gaaacattct tgggcacaaa ttggaataca actataactc acacaatgta
      481 tacatcatgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tagacacaac
      541 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      601 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      661 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      721 catggcatgg atgaactata caaatagggc gcgccacttc taaataagcg aatttcttat
      781 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
      841 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactcttt
//
