LOCUS       sequence-83740-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LacZ driven by the human Gfa2 promoter. LacZ can also 
            be replaced with the gene of interest for targeting transgene 
            expression to astrocytes in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Brenner M, Kisseberth WC, Su Y, Besnard F, Messing A
  TITLE     GFAP promoter directs astrocyte-specific expression in transgenic 
            mice.
  JOURNAL   J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
  PUBMED    8120611
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1994-03"; volume: "14(3 Pt 1)"; pages: "1030-7"
COMMENT     Sequence Label: pGfa2-nLac
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(139..161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 caggttggag aggagacgca tcacctccgc tgctcgcggg gatcctctag agtcgacgga
       61 tccggggaat tccccagtct caggatccac catggggccc aagaagaaac gcaaagtggg
      121 gagcatgggg gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
      301 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
      361 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
      421 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
      481 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
      541 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
      601 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
      661 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg
      721 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
      781 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
      841 agttcagatg tgcggcgagt tgcgtgacta ccctacgggt aacagttttc tttatggcag
      901 ggtgaaaacg caggtcgcca gcggcaccgc gccctttcgg cggtgaaatt atcgatgagc
      961 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaaactgtgg
     1021 agcgcc
//
