LOCUS       sequence-83741-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LacZ driven by the human Gfa2 promoter. LacZ can also 
            be replaced with the gene of interest for targeting transgene 
            expression to astrocytes in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Brenner M, Kisseberth WC, Su Y, Besnard F, Messing A
  TITLE     GFAP promoter directs astrocyte-specific expression in transgenic 
            mice.
  JOURNAL   J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
  PUBMED    8120611
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1994-03"; volume: "14(3 Pt 1)"; pages: "1030-7"
COMMENT     Sequence Label: pGfa2-nLac
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..183
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(204..224)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac
       61 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      121 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      181 acgacgggat cccccatgct ccccactttg cgtttcttct tgggccccat ggtggatcct
      241 gagactgggg aattccccgg atccgtcgac tctagaggat ccccgcgagc agcggaggtg
      301 atgcgtctcc tctccaacct gctctggctc tgctcgctcc tgggatgcga gggctttatg
      361 aaggagtggg ctagactggc gatgcccggg tgcccctggc aacaccccct ggcatagcct
      421 gagggggtgg ggttgggccg cagcccagct atggggagag ctcccctcac cccactgagg
      481 tcactgtgcc cagaggcaga gcttcctggg cacgccaact cctgcatgag gcaggtggta
      541 cctggagcca gaccatgggt ctggggccct gaccgctgaa gcaagaggac cctctctcta
      601 ggaaggtggg tcaagaaagg gttggttgga ccatgatgaa tacttacaat tattatgcat
      661 caattaaaaa ggaaggaaag ggctgagtgg ggtggctcat gcttgtaatc ccagcacttt
      721 gggaggctga ggctggagga ttgcttgagc ccagtaggtt gaggctgcag tgagctgtgt
      781 ttgcgccact ccagcctggg caacagagag agaccttgtc tcacaaaaaa aaaaaaaaaa
      841 aaagggaagg aagggctgta ccagatgacc tctgacctgc tttccaactt tttgatttca
      901 taacccaggc attatctcac tcagctgtaa gtgcctgcaa ggtgctggga atttggcgaa
      961 gaatgaa
//
