LOCUS       sequence-83745-e        7614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage sensor based on mUKG-mKOk FRET pair.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA
  TITLE     Fluorescent protein voltage probes derived from ArcLight that 
            respond to membrane voltage changes with fast kinetics.
  JOURNAL   PLoS One. 2013 Nov 27;8(11):e81295. doi: 
            10.1371/journal.pone.0081295. eCollection 2013.
  PUBMED    24312287
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-27"; volume: "8(11):e81295. doi"; pages: " 
            10.1371/journal.pone.0081295. eCollection 2013"
COMMENT     Sequence Label: Chicken Mermaid S188
FEATURES             Location/Qualifiers
     source          1..7614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             640..1317
                     /codon_start=1
                     /product="monomeric variant of Umikinoko-Green fluorescent 
                     protein"
                     /label=mUkG1
                     /translation="MSVIKEEMKIKLHMEGNVNGHAFVIEGDGKGKPYDGTQTLNLTVK
                     EGAPLPFSYDILTNAFQYGNRAFTKYPADIPDYFKQTFPEGYSWERTMSYEDNAICNVR
                     SEISMEGDCFIYKIRFDGKNFPPNGPVMQKKTLKWEPSTEMMYVRDGFLMGDVNMALLL
                     EGGGHHRCDFKTSYKAKKVVQLPDAHKIDHRIEILSHDRDYSKVKLYENAVARNSLLPS
                     QASK"
     CDS             1327..1983
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     polyA_signal    2013..2147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2252..2272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2257..2586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2437..2572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2499..2518
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2635..3660
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2635..3660
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3790..3911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3949..3965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3949..3965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3962..3984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3973..3989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3997..4003,4004..4021,4022..4027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4042..4063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4180..4197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4351..4939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4431..4450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5110..5901,5902..5970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5733..5752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5971..6075)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6101..6556)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6238..6259)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6450..6469
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6630..7614
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagctag cttgatttag gtgacactat agaatacaag
       61 cttatggaga cgactgtgag gtatgaacag gggtcagagc tcactaaaac ttcgagctct
      121 ccaacagcag atgagcccac gataaagatt gatgatggtc gtgatgaggg taatgaacaa
      181 gacagctgtt ccaataccat taggagaaaa atttccccgt ttgtgatgtc atttggattc
      241 agagtatttg gagttgtgct tatcattgta gacatcatag tggtgattgt ggatctggcc
      301 atcagtgaga agaaaagagg cattagagag attcttgaag gtgtttccct ggctatagca
      361 ctcttcttcc ttgttgatgt tctcatgaga gtgtttgttg aaggcttcaa gaactatttc
      421 cggtccaaac tgaatacttt ggatgcagtc atagtagtgg gcactctgct aattaatatg
      481 acctactcct tctctgacct tgctgccaca gatcagatgc cgcagatggt tactcttctt
      541 cgagttctga gaattgttat cttaataaga atatttcgcc tggcttcaca gaagaaacaa
      601 cttgaagtgg taaccagaag aatggtctct ggggatccca tgagtgtgat taaagaggaa
      661 atgaagatca agctgcatat ggaaggaaat gtaaacggtc atgcatttgt gattgaagga
      721 gatggaaaag gaaagcctta cgatgggaca cagactttaa acctgacagt gaaagaaggc
      781 gcacctctcc ctttttctta cgacatcttg acaaatgcgt tccagtacgg aaatagagca
      841 ttcactaaat atccagccga tataccagac tatttcaagc agacgtttcc cgaggggtat
      901 tcatgggaaa gaaccatgag ttatgaagac aacgccattt gcaacgtgag aagcgagatc
      961 agcatggaag gcgactgctt tatctataaa attcggtttg atggcaagaa ctttcccccc
     1021 aatggtccag ttatgcagaa gaaaactttg aagtgggaac catccactga gatgatgtac
     1081 gtgcgtgatg ggtttctgat gggtgatgtt aacatggctc tgttgcttga aggaggtggc
     1141 catcaccgat gtgacttcaa aacttcctac aaagcgaaaa aggttgtgca gttgccagat
     1201 gcccacaaga tcgaccatcg tatcgagatc ttgagccatg acagggatta cagcaaagtc
     1261 aagctgtatg agaatgcggt tgctcgcaat tctttgctgc caagtcaggc ttcgaagctc
     1321 gagaccatgg tgagtgtgat taaaccagag atgaagatga ggtactacat ggacggctcc
     1381 gtcaatgggc atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat
     1441 caagagatga cactacgcgt cacaatggcc gagggcgggc caatgccttt cgcgtttgac
     1501 ttagtgtcac acgtgttctg ttacggccac agagtattta ctaaatatcc agaagagata
     1561 ccagactatt tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc
     1621 gaagatggtg ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac
     1681 cacaaatcca aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa
     1741 agtgttgatt gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt
     1801 gatgttacga tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact
     1861 acttacaagg cggcaaaaga gattcttgaa atgccaggag accattacat cggccatcgc
     1921 ctcgtcagga aaaccgaagg caacattact gagcaggtag aagatgcagt agctcattcc
     1981 taatctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     2041 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2101 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2161 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttgatatccg
     2221 cggcgccaat gcattgggcc cggtaccctg tggaatgtgt gtcagttagg gtgtggaaag
     2281 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2341 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2401 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2461 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2521 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2581 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa
     2641 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     2701 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     2761 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     2821 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     2881 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     2941 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     3001 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     3061 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     3121 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     3181 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     3241 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     3301 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     3361 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     3421 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     3481 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     3541 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     3601 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     3661 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3901 aatgtatctt atcatgtctg tataccgtac gcgcttggcg taatcatggt catagctgtt
     3961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     6121 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     6181 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     6241 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     6301 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     6361 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     6421 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     6481 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6541 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6601 gtgcgggcct cttcgctatt acgccagtcg accatagcca attcaatatg gcgtatatgg
     6661 actcatgcca attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg
     6721 actcgtgcca attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg
     6781 caccatgcca attcaatatg gcggacttgg cactgtgcca actggggagg ggtctacttg
     6841 gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg
     6901 gcatggtgcc aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata
     6961 tccaatatgg ccctatgcca atatggctat tggccaggtt caatactatg tattggccct
     7021 atgccatata gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg
     7081 gcggtcccat ataccatata tggggcttcc taataccgcc catagccact cccccattga
     7141 cgtcaatggt ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg
     7201 tatatggcgc ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc
     7261 attgacgtca ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat
     7321 atccgttctc acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat
     7381 caatatctat taatagtaac ttggcaagta cattactatt ggaaggacgc cagggtacat
     7441 tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat
     7501 ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg
     7561 ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaac
//
