LOCUS       sequence-83746-e        1684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1684)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZFYVE9C-c009
FEATURES             Location/Qualifiers
     source          1..1684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga atcttattcc tgaagatggc cttcctccca ttctcatctc cactggtgta
      121 aaaggagact atgctgtgga agagaaacca tcacagattt cagtaatgca gcagttggag
      181 gatggtggcc ctgacccact tgtatttgtt ttaaatgcaa atttgttgtc aatggttaaa
      241 attgtaaatt atgtgaacag gaagtgctgg tgtttcacaa ccaagggaat gcatgcagtg
      301 ggtcagtctg agatagtcat tcttctacag tgtttaccgg atgaaaagtg tttgccaaag
      361 gatatcttta atcactttgt gcagctttat cgggatgctc tggcagggaa tgtggtgagc
      421 aacttgggac attccttctt cagtcaaagt ttccttggca gtaaagaaca tggtggattc
      481 ttatatgtga catctaccta ccagtcactg caagacctag tactcccaac cccaccttac
      541 ttgtttggga ttcttatcca gaaatgggaa actccttggg ctaaagtatt tcctatccgt
      601 ctgatgttga gacttggagc tgaatatcga ctttatccat gcccactatt cagtgtcaga
      661 tttcggaagc cattgtttgg agagacgggg cataccatca tgaatcttct tgcagacttc
      721 agaaattacc agtatacctt gccagtagtt caaggtttgg tggttgatat ggaagttcgg
      781 aaaactagca tcaaaattcc cagcaacaga tacaatgaga tgatgaaagc catgaacaag
      841 tccaatgagc atgtcctggc aggaggtgcc tgcttcaatg aaaaggcaga ctctcatctt
      901 gtgtgtgtac agaatgatga tggaaactat cagacccagg ctatcagtat tcacaatcag
      961 cccagaaaag tgactggtgc cagtttcttt gtgttcagtg gcgctctgaa atcctcttct
     1021 ggataccttg ccaagtccag tattgtggaa gatggtgtta tggtccagat tactgcagag
     1081 aacatggatt ccttgaggca ggcactgcga gagatgaagg acttcaccat cacctgtggg
     1141 aaggcggacg cggaggaacc ccaggagcac atccacatcc agtgggtgga tgatgacaag
     1201 aacgttagca agggtgtcgt aagtcctata gatgggaagt ccatggagac tataacaaat
     1261 gtgaagatat tccatggatc agaatataaa gcaaatggaa aagtaatcag atggacagag
     1321 gtgtttttcc tagaaaacga tgaccagcac aattgcctca gtgatcctgc agatcacagt
     1381 agattgactg agcatgttgc caaagctttt tgccttgctc tctgtcctca cctgaaactt
     1441 ctgaaggaag atggaatgac caaactggga ctacgtgtga cacttgactc agatcaggtt
     1501 ggctatcaag cagggagcaa tggccagccc cttccctcgc agtacatgaa tgatctggac
     1561 agcgccttgg tgccggtgat ccatggaggg gcctgccagc ttagtgaggg ccccgttgtc
     1621 atggaactca tcttttatat tctggaaaac atcgtatgac agtaaaggtg gatacggatc
     1681 cgaa
//
