LOCUS       sequence-83749-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GLS2A-c007
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga ttcctgattt tgaggagttc acgggccatg tggatcgcat ctttgaggat
      121 gtcaaagagc tcactggagg caaagtggca gcctacatcc ctcagctggc caagtcaaac
      181 ccagacctgt ggggtgtctc cctgtgcact gtggatggtc aacggcactc tgtgggccac
      241 acaaagatcc ccttctgcct gcagtcctgt gtgaagcccc tcacctatgc catctccata
      301 agcaccctag gcactgacta cgtgcacaag tttgtgggca aagagccaag tggcctgcgc
      361 tacaacaagc tctccctcaa tgaggaagga atcccccata accccatggt caatgctggt
      421 gccattgttg tcagctccct gatcaagatg gactgtaaca aagcagagaa gtttgatttt
      481 gtgttgcagt atctcaacaa aatggctggg aatgaataca tgggtttcag caatgccaca
      541 ttccagtcag agaaggaaac aggggatcgg aattatgcca tcggctatta tctcaaggaa
      601 aagaagtgct ttcctaaggg ggtggacatg atggctgccc ttgatctcta cttccagctg
      661 tgttctgtgg aggtcacttg tgaatcaggc agtgtcatgg cagccaccct cgccaacggt
      721 gggatctgcc ccatcacagg cgagagtgtg ctgagtgctg aagcagtgcg caacaccctc
      781 agcctcatgc attcctgcgg catgtatgac ttctctggcc agtttgcctt ccacgtgggc
      841 ctgccagcca agtcagctgt atcaggagcc atcctcctgg tggtacccaa tgtcatggga
      901 atgatgtgcc tgtcaccccc attggacaag ctggggaaca gccatagggg gaccagcttc
      961 tgccagaagt tggtgtctct cttcaatttc cacaactatg acaacctgag gcactgtgct
     1021 cggaagttag acccacggcg tgaagggtga cagtaaaggt ggatacggat ccgaa
//
