LOCUS       sequence-83755-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JMJD1BA-c068
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1070..1087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1088..1111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgacttctca ctcctggctt tgtgatggga ggcttctgtg
       61 tctccatgac cccagcaaca aaaacaattg gaagatcttc cgggagtgtt ggaagcaagg
      121 tcagccagtg ctggtttcgg gggtacataa aaagctcaag tctgagctct ggaagccaga
      181 agcctttagc caggaatttg gagaccagga tgtagacttg gtgaactgca ggaactgtgc
      241 tataatttcc gatgtgaaag ttcgggattt ctgggatggt ttcgagatca tatgcaaacg
      301 actacggtca gaagatgggc agccaatggt gctcaaactc aaggactggc ctcctgggga
      361 agattttcga gacatgatgc caaccaggtt tgaagatctg atggagaacc ttcctctgcc
      421 agaatatacc aaacgagatg gcaggctcaa tctggcctct aggctaccta gctactttgt
      481 aaggcctgat ctgggcccca agatgtacaa cgcctatggg ttgataacag cagaagatag
      541 aagagttggt acaacaaatc ttcacttaga tgtgtctgat gctgttaatg tgatggtgta
      601 tgttgggatt cccatcgggg agggtgctca tgatgaagag gtactcaaga caattgacga
      661 gggagatgcc gatgaggtga cgaagcagag gattcatgat ggaaaagaga agccaggtgc
      721 tttatggcac atctatgcag ccaaggatgc agagaagatc cgggagctgc tccgaaaggt
      781 tggagaagaa caaggccaag agaacccccc tgatcatgac ccaattcatg accaaagttg
      841 gtacctggac cagaccctcc gtaagcgact ctatgaggag tatggcgtgc aaggctgggc
      901 tattgtgcag ttcctaggtg atgctgtttt catacctgct ggagccccac accaggttca
      961 caatctatac agttgcataa aagtagcaga agactttgta tctccagaac atgtaaagca
     1021 ctgtttccgc ctgactcagg aattcgcagg gaacctctac ttccaatcgc accatcatca
     1081 ccaccatgat tacaaggatg acgacgataa gtgaggatcc
//
