LOCUS       sequence-83759-e        2139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2139)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BLMA-c800
FEATURES             Location/Qualifiers
     source          1..2139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2065..2085
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2092..2136
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt ccagaaatct gaaacatgag cgtttccaaa gtcttagttt tcctcataca
      121 aaggaaatga tgaagatttt tcataaaaaa tttggcctgc ataattttag aactaatcag
      181 ctagaggcga tcaatgctgc actgcttggt gaagactgtt ttatcctgat gccgactgga
      241 ggtggtaaga gtttgtgtta ccagctccct gcctgtgttt ctcctggggt cactgttgtc
      301 atttctccct tgagatcact tatcgtagat caagtccaaa agctgacttc cttggatatt
      361 ccagctacat atctgacagg tgataagact gactcagaag ctacaaatat ttacctccag
      421 ttatcaaaaa aagacccaat cataaaactt ctatatgtca ctccagaaaa gatctgtgca
      481 agtaacagac tcatttctac tctggagaat ctctatgaga ggaagctctt ggcacgtttt
      541 gttattgatg aagcacattg tgtcagtcag tggggacatg attttcgtca agattacaaa
      601 agaatgaata tgcttcgcca gaagtttcct tctgttccgg tgatggctct tacggccaca
      661 gctaatccca gggtacagaa ggacatcctg actcagctga agattctcag acctcaggtg
      721 tttagcatga gctttaacag acataatctg aaatactatg tattaccgaa aaagcctaaa
      781 aaggtggcat ttgattgcct agaatggatc agaaagcacc acccatatga ttcagggata
      841 atttactgcc tctccaggcg agaatgtgac accatggctg acacgttaca gagagatggg
      901 ctcgctgctc ttgcttacca tgctggcctc agtgattctg ccagagatga agtgcagcag
      961 aagtggatta atcaggatgg ctgtcaggtt atctgtgcta caattgcatt tggaatgggg
     1021 attgacaaac cggacgtgcg atttgtgatt catgcatctc tccctaaatc tgtggagggt
     1081 tactaccaag aatctggcag agctggaaga gatggggaaa tatctcactg cctgcttttc
     1141 tatacctatc atgatgtgac cagactgaaa agacttataa tgatggaaaa agatggaaac
     1201 catcatacaa gagaaactca cttcaataat ttgtatagca tggtacatta ctgtgaaaat
     1261 ataacggaat gcaggagaat acagcttttg gcctactttg gtgaaaatgg atttaatcct
     1321 gatttttgta agaaacaccc agatgtttct tgtgataatt gctgtaaaac aaaggattat
     1381 aaaacaagag atgtgactga cgatgtgaaa agtattgtaa gatttgttca agaacatagt
     1441 tcatcacaag gaatgagaaa tataaaacat gtaggtcctt ctggaagatt tactatgaat
     1501 atgctggtcg acattttctt ggggagtaag agtgcaaaaa tccagtcagg tatatttgga
     1561 aaaggatctg cttattcacg acacaatgcc gaaagacttt ttaaaaagct gatacttgac
     1621 aagattttgg atgaagactt atatatcaat gccaatgacc aggcgatcgc ttatgtgatg
     1681 ctcggaaata aagcccaaac tgtactaaat ggcaatttaa aggtagactt tatggaaaca
     1741 gaaaattcca gcagtgtgaa aaaacaaaaa gcgttagtag caaaagtgtc tcagagggaa
     1801 gagatggtta aaaaatgtct tggagaactt acagaagtct gcaaatctct ggggaaagtt
     1861 tttggtgtcc attacttcaa tatttttaat accgtcactc tcaagaagct tgcagaatct
     1921 ttatcttctg atcctgaggt tttgcttcaa attgatggtg ttactgaaga caaactggaa
     1981 aaatatggtg cggaagtgat ttcagtatta cagaaatact ctgaatggac atcgccagct
     2041 gaagacagtt ccccagggat gtccgagaat ttgtatttcc aatccggtgg cggtctgaac
     2101 gacatcttcg aggctcagaa aatcgaatgg cacgaatga
//
