LOCUS       sequence-83760-e        1321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1321)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MYO5AA-c010
FEATURES             Location/Qualifiers
     source          1..1321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggagaacat atccccagga cagatcattg atgaacccat ccgaccagtc
      121 aacattccca ggaaagaaaa ggatttccaa gggatgctgg aatacaagaa ggaggatgag
      181 caaaaacttg ttaagaacct gattctggaa ctgaagccac gtggtgtagc agtcaatttg
      241 attccaggat taccggcata tatcctgttc atgtgtgttc gacatgctga ctacctgaat
      301 gatgatcaga aagtaaggtc gttgctaaca tcaacaatta acagcatcaa aaaagtattg
      361 aagaaaagag gtgatgattt tgaaaccgtc tccttctggc tctctaacac atgccgattt
      421 ttgcactgct tgaaacagta cagtggagaa gagggcttta tgaagcacaa cacatctcgc
      481 cagaatgaac actgcctcac caattttgac ctggctgagt atcggcaggt gctgagtgac
      541 ttggccattc agatctacca gcagctcgtg cgggtgttag agaacatcct tcagccaatg
      601 attgtctcag gcatgctgga acatgaaacg attcagggcg tgtctggggt gaagcccaca
      661 gggttgagaa agcgaacctc cagtatcgcc gatgagggca cctacacact ggactccatc
      721 ctccggcagc tcaactcctt ccactcggtc atgtgtcagc atggcatgga ccctgaactg
      781 atcaagcagg tggtcaagca gatgttctac atcatagggg ccatcaccct gaacaacctt
      841 ctcctgcgga aggacatgtg ctcctggagt aaaggcatgc agatcaggta caatgtcagt
      901 caactggaag aatggctgcg tgacaagaat ctgatgaata gtggggctaa agaaaccctg
      961 gaacctctca ttcaggctgc tcaacttttg caagtgaaaa agaaaacaga tgatgatgca
     1021 gaagccattt gttctatgtg caatgcttta actactgccc agattgtgaa agtgttgaat
     1081 ttgtatactc cagttaatga gtttgaagaa agagtctctg tgtcgttcat tcgtactata
     1141 cagatgcgtt tacgagacag gaaagactct ccccagctgc tcatggatgc taaacacatc
     1201 tttcctgtca cctttccttt caacccatct tccctcgcac tagaaaccat ccagattcca
     1261 gccagcctcg gcctgggctt catttcacgg gtctgacagt aaaggtggat acggatccga
     1321 a
//
