LOCUS       sequence-83762-e        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBSA-c032
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc cttctgagac cccccaggca gaagtggggc ccacaggctg cccccaccgc
      121 tcagggccac actcggcgaa ggggagcctg gagaaggggt ccccagagga taaggaagcc
      181 aaggagcccc tgtggatccg gcccgatgct ccgagcaggt gcacctggca gctgggccgg
      241 cctgcctccg agtccccaca tcaccacact gccccggcaa aatctccaaa aatcttgcca
      301 gatattctga agaaaatcgg ggacacccct atggtcagaa tcaacaagat tgggaagaag
      361 ttcggcctga agtgtgagct cttggccaag tgtgagttct tcaacgcggg cgggagcgtg
      421 aaggaccgca tcagcctgcg gatgattgag gatgctgagc gcgacgggac gctgaagccc
      481 ggggacacga ttatcgagcc gacatccggg aacaccggga tcgggctggc cctggctgcg
      541 gcagtgaggg gctatcgctg catcatcgtg atgccagaga agatgagctc cgagaaggtg
      601 gacgtgctgc gggcactggg ggctgagatt gtgaggacgc ccaccaatgc caggttcgac
      661 tccccggagt cacacgtggg ggtggcctgg cggctgaaga acgaaatccc caattctcac
      721 atcctagacc agtaccgcaa cgccagcaac cccctggctc actatgacac caccgctgat
      781 gagatcctgc agcagtgtga tgggaagctg gacatgctgg tggcttcagt gggcacgggc
      841 ggcaccatca cgggcattgc caggaagctg aaggagaagt gtcctggatg caggatcatt
      901 ggggtggatc ccgaagggtc catcctcgca gagccggagg agctgaacca gacggagcag
      961 acaacctacg aggtggaagg gatcggctac gacttcatcc ccacggtgct ggacaggacg
     1021 gtggtggaca agtggttcaa gagcaacgat gaggaggcgt tcacctttgc ccgcatgctg
     1081 atcgcgcaag aggggctgct gtgcggtggc agtgctggca gcacggtggc ggtggccgtg
     1141 aaggctgcgc aggagctgca ggagggccag cgctgcgtgg tcattctgcc cgactcagtg
     1201 cggaactaca tgaccaagtt cctgagcgac aggtggatgc tgcagaaggg ctttctgaag
     1261 gaggaggacc tcacggagaa gaagccctgg tggtggcacc tccgtgttca ggagctgggc
     1321 ctgtcagccc cgctgaccgt gctcccgacc atcacctgtg ggcacaccat cgagatcctc
     1381 cgggagaagg gcttcgacca ggcgcccgtg gtggatgagg cgggggtaat cctgggaatg
     1441 gtgacgcttg ggaacatgct ctcgtccctg cttgccggga aggtgcagcc gtcagaccaa
     1501 gttggcaaag tcatctacaa gcagttcaaa cagatccgcc tcacggacac gctgggcagg
     1561 ctctcgcaca tcctggagat ggaccacttc gccctggtgg tgcacgagca gcggcagatg
     1621 gtgttcgggg tggtcaccgc cattgacttg ctgaacttcg tggccgccca ggagcgggac
     1681 cagaagtgac agtaaaggtg gatacggatc cgaa
//
