LOCUS       sequence-83769-e        1991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1991)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DPYSL3A-c010
FEATURES             Location/Qualifiers
     source          1..1991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..204
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtgtggata acaagttcaa caaggagcgt
       61 cgaagagctc gccgtgaaat tcgccatctg ccgaacctga accgcgaaca gcgtcgcgca
      121 tttattcgca gcctgcgcga tgatccgagc cagagcgcga acctgctggc ggaagcgaag
      181 aagctgaacg atgcgcagcc gaagggtaca gagaacctgt acttccaatc catgtcctac
      241 caaggcaaga agaacatccc gcggatcacg agtgaccgtc tccttatcaa gggaggcaga
      301 atcgtcaatg atgatcagtc cttttatgct gatatttaca tggaagatgg cttaataaaa
      361 caaattggag acaatctgat tgttcctgga ggagtgaaga ccattgaagc caatgggaag
      421 atggtgatcc ctggaggcat cgatgtccat actcactacc agatgccata taagggaatg
      481 accacagtag atgacttctt ccaagggaca aaggcggctt tagcaggtgg caccaccatg
      541 atcattgacc atgtggtgcc tgagcctgag tccagcctga ctgaggccta tgagaaatgg
      601 agagagtggg ctgatgggaa gagttgctgt gactatgccc tgcatgtgga catcacccac
      661 tggaatgaca gcgtcaagca ggaagtgcag aacctcatca aggacaaagg ggttaactcc
      721 ttcatggttt atatggctta taaggatttg tatcaagtat ctaacacaga gctctatgag
      781 atcttcacct gcctgggaga gctgggggcc attgctcaag ttcatgctga gaatggggat
      841 atcattgccc aggagcaaac ccgcatgttg gaaatgggga taactggccc agaaggccat
      901 gtactgagca ggccagaaga gctggaagct gaggctgtgt tccgtgccat caccattgcc
      961 agccaaacca attgccctct ctacgtcaca aaggtcatga gcaagagtgc agctgacctc
     1021 atctcacaag ccaggaaaaa aggaaatgta gtctttggtg agcccatcac tgccagcctc
     1081 ggcatagatg gaacccatta ttggagcaag aactgggcca aggcggctgc atttgtgaca
     1141 tccccacccc tgagccctga cccaactact ccggactaca tcaactcctt gctggccagc
     1201 ggggatctgc agctatctgg gagtgcccac tgcaccttca gcactgccca gaaagcaatt
     1261 gggaaggaca acttcacagc cattcctgag ggcaccaatg gtgtggagga gcggatgtct
     1321 gtcatctggg acaaggctgt ggccacaggg aaaatggacg aaaaccagtt cgtggctgtg
     1381 acaagcacaa acgctgccaa gatcttcaac ctgtatcccc gcaagggaag aatatctgtg
     1441 ggttctgaca gcgacctcgt catctgggat ccagatgctg tgaagatcgt ctctgccaag
     1501 aaccaccagt ctgcggcaga gtacaacatc tttgaaggga tggagctgcg cggggctcct
     1561 ctggttgtca tctgccaggg caagatcatg ctggaagatg gcaacctgca cgtgacccag
     1621 ggggctggcc gcttcatacc ctgcagcccg ttctccgact atgtctacaa gcgcattaaa
     1681 gcacggagga agatggcaga cctgcatgcc gtcccaaggg gcatgtacga tgggcctgtg
     1741 tttgacctga ccaccacccc caaaggtggc acccccgcag gctctgctcg gggctctcct
     1801 actcggccga acccacctgt gaggaatctt catcagtcgg gatttagcct gtcaggcact
     1861 caagtggatg agggggttcg ctcagccagc aagcgcatcg tggcgccccc aggcggccgt
     1921 tctaatatca catctctgag ttgacagtaa aggtggatac ggatccgaat tcgagctccg
     1981 tcgacaagct t
//
