LOCUS       sequence-83777-e        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZADH1A-c501
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1054..1074
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1075..1092
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1093..1116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgatgattg ttcaaagagt ggtattgaat tctcgacctg gaaaaaatgg taatccagtg
       61 gcagagaatt tccgaatgga agaagtctat ttaccagata atattaatga aggacaagta
      121 caagttagaa ctctttatct ttctgtggat ccttacatgc gttgtagaat gaatgaagac
      181 actggcactg attatataac accttggcag ctatctcaag tcgttgatgg tggaggtatt
      241 ggaattatag aagaaagcaa acacacaaat ttgactaaag gcgattttgt gacttctttc
      301 tattggccct ggcaaaccaa ggttattctg gatggaaata gccttgaaaa ggtagaccca
      361 caacttgtgg atggacacct ttcatatttt cttggagcta taggtatgcc tggtttgact
      421 tccttgattg ggatacagga aaaaggtcat ataactgctg gatctaataa gacaatggtt
      481 gtcagtgggg ccgcaggtgc ctgtggatct gtggctgggc agattggcca tttcttaggt
      541 tgttccagag tggtgggaat ttgtggaaca catgagaaat gcatcctctt gacctcagaa
      601 ctgggctttg atgctgcaat taattataaa aaagacaatg tggcagaaca gctccgtgaa
      661 tcatgcccag ctggagtgga tgtttatttt gacaatgttg gtggtaacat cagtgataca
      721 gtgataagtc agatgaatga gaacagccac atcatcctgt gtggtcaaat ttctcagtac
      781 aacaaagatg tgccttatcc tcccccgcta tcccctgcta tagaggcaat ccagaaagaa
      841 agaaacatca caagggaaag atttctggta ttaaattata aagacaaatt tgagcctggc
      901 attctacagc tgagtcagtg gtttaaagaa ggaaagctaa agattaaaga gacggtaata
      961 aatgggttgg aaaacatggg agctgcattc cagtccatga tgacaggagg taacattgga
     1021 aagcagatag tttgcatttc agaagaaatc gcagagaacc tctacttcca atcgcaccat
     1081 catcaccacc atgattacaa ggatgacgac gataagtgag gatcc
//
