LOCUS       sequence-83779-e         535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 535)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HTD2A-c022
FEATURES             Location/Qualifiers
     source          1..535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tgccagtgct gaccctgcag cactttcagc atatgcacat caaagttgga
      121 gaccgcgctg aacttaggag ggccttcaca cagactgatg tggctacctt ctcagaatta
      181 acaggggatg tcaatccttt gcatttgaat gaagactttg caaaacacac caagtttgga
      241 aatacaattg tacatggagt tttgatcaac ggacttatct cagctctcct aggaactaaa
      301 atgccagggc caggctgtgt atttctttcc caggaaatta gctttccagc ccctttatat
      361 attggagaag ttgttttagc ttctgcagaa gtgaaaaagc tgaagcggtt cattgctatt
      421 attgcagtgt catgttctgt aatagaaagt aaaaagactg ttatggaagg ctgggttaaa
      481 gttatggttc cagaagcttc caaatcctga cagtaaaggt ggatacggat ccgaa
//
