LOCUS       sequence-83780-e        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HPDA-c103
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1165..1185
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1186..1203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1204..1227
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcaa agcctgagag aggccgattc ctccacttcc actctgtgac cttctgggtt
       61 ggcaacgcca agcaggccgc gtcattctac tgcagcaaga tgggctttga acctctagcc
      121 tacaggggcc tggagaccgg ttcccgggag gtggtcagcc atgtaatcaa acaagggaag
      181 attgtgtttg tcctctcctc agcgctcaac ccctggaaca aagagatggg cgatcacctg
      241 gtgaaacacg gtgacggagt gaaggacatt gcgttcgagg tggaagattg tgactacatc
      301 gtgcagaaag cacgggaacg gggcgccaaa atcatgcggg agccctgggt agagcaagac
      361 aagtttggga aggtgaagtt tgctgtgctg cagacgtatg gggacaccac acacaccctg
      421 gtggagaaga tgaactacat cggccaattc ttgcctggat atgaggcccc agcgttcatg
      481 gaccccctac ttcctaaact gcccaaatgc agtctggaga tgatcgacca cattgtggga
      541 aaccagcctg atcaggagat ggtgtccgcc tccgaatggt acctgaaaaa cctgcagttc
      601 caccgcttct ggtccgtgga tgacacgcag gtgcacacgg aatatagctc tctgcgatcc
      661 attgtggtgg ccaactatga agagtccatc aagatgccca tcaatgagcc agcgcctggc
      721 aagaagaagt cccagatcca ggaatatgtg gactataacg ggggcgctgg ggtccagcac
      781 atcgctctca agaccgaaga catcatcaca gcgattcgcc acttgagaga gagaggcctg
      841 gagttcttat ctgttccctc cacgtactac aaacaactgc gggagaagct gaagacggcc
      901 aagatcaagg tgaaggagaa cattgatgcc ctggaggagc tgaaaatcct ggtggactac
      961 gacgagaaag gctacctcct gcagatcttc accaaaccgg tgcaggaccg gcccacgctc
     1021 ttcctggaag tcatccagcg ccacaaccac cagggttttg gagccggcaa cttcaactca
     1081 ctgttcaagg ctttcgagga ggagcagaac ctgcggggta acctcaccaa catggagacc
     1141 aatggggtgg tgcccggcat ggcagagaac ctctacttcc aatcgcacca tcatcaccac
     1201 catgattaca aggatgacga cgataagtga ggatcc
//
