LOCUS       sequence-83788-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEK1A-c011
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg agaagtatgt tagactacag aagattggag aaggttcatt tggaaaagcc
      121 attcttgtta aatctacaga agatggcaga cagtatgtta tcaaggaaat taacatctca
      181 agaatgtcca gtaaagaaag agaagaatca aggagagaag ttgcagtatt ggcaaacatg
      241 aagcatccaa atattgtcca gtatagagaa tcatttgaag aaaatggctc tctctacata
      301 gtaatggatt actgtgaggg aggggatctg tttaagcgaa taaatgctca gaaaggcgtt
      361 ttgtttcaag aggatcagat tttggactgg tttgtacaga tatgtttggc cctgaaacat
      421 gtacatgata gaaaaattct tcatcgagac attaaatctc agaacatatt tttaactaaa
      481 gatggaacag tacaacttgg agattttgga attgctagag ttcttaatag tactgtagag
      541 ctggctcgag cttgcatagg gaccccatac tacttgtcac ctgaaatctg tgaaaacaaa
      601 ccttacaata ataaaagtga catttgggct ctggggtgtg tcctttatga gctgtgtaca
      661 cttaaacatg cttttgaagc tggcagtatg aaaaacctgg tactgaagat aatatctgga
      721 tcttttccac ctgtgtcttt gcattattcc tatgatctcc gcagtttggt gtctcagtta
      781 tttaaaagaa atcctaggga tagaccatca gtcaactcca tattggagaa aggttttata
      841 gccaaacgca ttgaaaagtt tctctctcct cagcttattg cagaagaatt ttgtctaaaa
      901 acattttcga agtttggatc acagcctata ccagctaaaa gaccagcttc aggacaaaac
      961 tcgatttctg ttatgcctgc tcagaaaatt acaaagcctg ccgctaaata tggaatacct
     1021 ttagcatata agaaatatgg agataaaaaa tgacagtaaa ggtggatacg gatccgaa
//
