LOCUS       sequence-83791-e        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JMJD1CA-c257
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1159..1179
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1180..1197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgataccac attcttggat ctgtgagaag catattttat ggcttaagga ttataagaat
       61 agcagtaatt ggaagctttt caaagaatgt tggaaacaag gacagcctgc agtggtttct
      121 ggtgtgcata agaaaatgaa cattagccta tggaaggcgg aatcaattag tcttgatttt
      181 ggagaccacc aagctgatct cctgaactgc aaagatagca tcatttcaaa tgccaatgtt
      241 aaggaattct gggatggttt tgaagaagtt tcaaaacggc agaaaaacaa gagtggagaa
      301 acagttgttt taaaattgaa agactggcct tcaggagaag acttcaagac tatgatgcca
      361 gcaagatacg aagatctttt aaaaagtctg ccattgccag aatattgtaa tccagaagga
      421 aaattcaatt tggcctctca tttgccagga ttttttgtac gtcctgatct aggacccagg
      481 ttgtgcagtg cctatggtgt agttgctgct aaagatcatg atataggaac aacaaatctc
      541 catattgaag tttctgatgt tgtaaatata ctagtttatg ttggcatagc aaaaggaaat
      601 ggcattctct caaaagcagg aattctcaag aaatttgagg aagaagattt ggatgacatt
      661 ttaaggaaaa gattgaatga ctcaagtgaa atacctggtg ctctgtggca tatttatgct
      721 gggaaagatg ttgacaagat aagggaattt cttcaaaaga tttcaaaaga acaaggcctt
      781 gaagttctac cagaacatga tccaatacgt gaccaaagtt ggtatgtgaa caaaaagctc
      841 cgtcaaaggc tgcttgaaga atatggagtc agaacctgta ctcttattca gttccttggt
      901 gatgctattg ttttgccagc gggagcactt catcaggttc agaattttca cagctgtatt
      961 caggtaactg aagattttgt gtctccagaa catcttgtag agtcatttca tttaacacag
     1021 gaactgagac ttttgaagga agaaatcaat tatgatgata aactacaggt taaaaatatt
     1081 ttgtatcatg cagtcaaaga aatggtgaga gccttgaaga tacacgagga tgaagtagag
     1141 gatatggaag aaaatgcaga gaacctctac ttccaatcgc accatcatca ccaccatgat
     1201 tacaaggatg acgacgataa gtgaggatcc
//
