LOCUS       sequence-83794-e        1285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1285)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MYO5BA-c002
FEATURES             Location/Qualifiers
     source          1..1285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tcaacaggca ggtcacggtc cagcggaaag agaaggattt ccagggcatg
      121 ctggagtacc acaaagagga cgaggccctc ctcatccgga acctggtgac agacttgaag
      181 ccccagatgc tgtcgggcac agtgccctgt ctccccgcct acatcctcta catgtgcatc
      241 cggcacgcgg actacaccaa cgacgatctc aaggtgcact ccctgctgac ctccaccatc
      301 aacggcatta agaaagtcct gaaaaagcac aatgatgact ttgagatgac gtcattctgg
      361 ttatccaaca cctgccgcct tcttcactgt ctgaagcagt acagcgggga tgagggcttc
      421 atgactcaga acactgcaaa gcagaatgaa cactgtctta agaattttga cctcaccgaa
      481 taccgtcagg tgctgagtga cctttccatt cagatctacc agcagctcat taaaattgcc
      541 gagggcgtgt tacagccgat gatagtttct gccatgttgg aaaatgagag cattcagggt
      601 ctatctggtg tgaagcccac cggctaccgg aagcgctcct ccagcatggc agatggggat
      661 aactcatact gcctggaagc tatcatccgc cagatgaatg cctttcatac agtcatgtgt
      721 gaccagggct tggaccctga gatcatcctg caggtattca aacagctctt ctacatgatc
      781 aacgcagtga ctcttaacaa cctgctcttg cggaaggacg tctgctcttg gagcacaggc
      841 atgcaactca ggtacaatat aagtcagctt gaggagtggc ttcggggaag aaaccttcac
      901 cagagtggag cagttcagac catggaacct ctgatccaag cagcccagct cctgcaatta
      961 aagaagaaaa cccaggagga cgcagaggct atctgctccc tgtgtacctc cctcagcacc
     1021 cagcagattg tcaaaatttt aaacctttat actcccctga atgaatttga agaacgggta
     1081 acagtggcct ttatacgaac aatccaggca caactacaag agcggaatga ccctcagcaa
     1141 ctgctattag atgccaagca catgtttcct gttttgtttc catttaatcc atcttctcta
     1201 accatggact caatccacat cccagcgtgt ctcaatctgg aattcctcaa tgaagtctga
     1261 cagtaaaggt ggatacggat ccgaa
//
