LOCUS       sequence-83797-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASB9A-c009
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             787..807
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             808..825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             826..849
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtctgatt ggtctcctat gcatgaagct gcaatccacg gacatcagct gtctctgagg
       61 aacctcatca gccaggggtg ggctgtgaac atcatcacgg cagatcatgt ttccccactc
      121 catgaagcct gtcttggagg tcatctctct tgtgtgaaga ttttattaaa gcatggagct
      181 caggtgaatg gtgtgacagc agactggcac actccactgt ttaatgcttg tgtcagcggc
      241 agctgggatt gtgtgaattt gcttctgcag cacggagcca gcgttcaacc tgagagtgat
      301 ctggcatccc ccatccatga agctgctagg agaggccacg tggagtgtgt caactctctt
      361 atagcttatg ggggcaacat tgaccataag atcagccacc tgggcactcc actctatttg
      421 gcttgtgaaa accaacagag agcctgtgtc aagaagcttc tggagtcagg agcggacgtg
      481 aaccaaggga aaggtcagga ttccccactt catgcagtgg ccaggacagc cagtgaagag
      541 ctggcctgcc tgctcatgga ttttggagcg gacacccagg ccaagaatgc tgaaggcaaa
      601 cgtcctgtgg agctggtgcc tccagagagc cccttggccc agctcttctt ggagagagaa
      661 gggccccctt ctttgatgca gttatgccgc cttagaattc ggaagtgttt tggaatccag
      721 cagcatcata agataaccaa actcgtcctc ccagaggatc tgaaacagtt tctcctacat
      781 cttgcagaga acctctactt ccaatcgcac catcatcacc accatgatta caaggatgac
      841 gacgataagt gaggatcc
//
