LOCUS       sequence-83798-e         418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 418)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP1AA-c001
FEATURES             Location/Qualifiers
     source          1..418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             join(67..69,70..393)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
      121 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
      181 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
      241 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
      301 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
      361 gtcttcgatg tggagcttct aaaactggaa tgacagtaaa ggtggatacg gatccgaa
//
