LOCUS       sequence-83800-e        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CUL3A-c012
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1168..1188
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1189..1206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1207..1230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgagcaacc tgtccaaggg taccggcagt cgtaaggata ccaaaatgcg tatccgcgcc
       61 ttcccgatga ctatggacga aaaatacgtc aattcgattt gggatctgct gaagaacgct
      121 atccaggaga ttcagcgtaa aaacaattct ggtctgtcat ttgaagagct gtaccgcaac
      181 gcctatacta tggtgctgca caagcatggt gaaaaactgt ataccggcct gcgtgaagtg
      241 gtcaccgagc acctgatcaa taaggtgcgc gaggatgtcc tgaacagcct gaataataac
      301 ttcctgcaga ccctgaatca ggcctggaac gaccatcaga ccgctatggt gatgatccgt
      361 gacattctga tgtacatgga tcgcgtttat gtgcagcaga ataacgtcga aaatgtttac
      421 aacctgggtc tgatcatttt tcgtgaccag gttgtgcgct atggctgcat ccgtgaccac
      481 ctgcgccaga ccctgctgga tatgattgcc cgtgaacgca agggcgaggt cgttgatcgt
      541 ggcgccatcc gcaacgcttg tcagatgctg atgattctgg gtctggaagg ccgttctgtc
      601 tacgaagagg acttcgaggc gccctttctg gaaatgagtg cagagttctt tcagatggaa
      661 tcgcagaaat tcctggcgga aaacagcgca tcagtttata tcaagaaagt ggaagctcgt
      721 atcaacgaag agattgagcg cgttatgcat tgcctggata agtcaaccga agagccaatt
      781 gtgaaagtgg tcgaacgcga gctgatctct aagcacatga aaaccattgt cgaaatggaa
      841 aacagcggcc tggttcatat gctgaagaac ggtaaaaccg aagacctggg ctgtatgtac
      901 aagctgttca gccgtgtgcc gaatggtctg aaaaccatgt gcgaatgtat gagcagctat
      961 ctgcgtgagc agggcaaggc gctggtgtcc gaagagggtg aaggcaaaaa ccctgtggac
     1021 taccgccagg gtctggacga tctgaagagc cgtttcgacc gctttctgct ggagtccttc
     1081 aataacgatc gtctgtttaa acagaccatt gcaggcgact tcgaatactt tctgaatctg
     1141 aacagtcgct cgcccgagta tctggcagag aacctctact tccaatcgca ccatcatcac
     1201 caccatgatt acaaggatga cgacgataag tgaggatcc
//
