LOCUS       sequence-83801-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AK5A-c001
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gaggtttcat ggaagatttg agaaagtgta aaattatttt cataattggt
      121 ggtcctggct ctggcaaagg cacacagtgt gaaaagctgg tggaaaaata tggatttaca
      181 catctctcaa ctggcgagct cctgcgtgag gaactggcat cagaatctga aagaagcaaa
      241 ttgatcagag acattatgga acgtggagac ctggtgccct caggcatcgt tttggagctc
      301 ctgaaggagg ccatggtggc cagcctcggg gacaccaggg gcttcctgat tgacggctat
      361 cctcgggagg tgaagcaagg ggaagagttc ggacgcagga ttggagaccc acagttggtg
      421 atctgtatgg actgctcggc agacaccatg accaaccgcc ttctccaaag gagccggagc
      481 agcctgcctg tggacgacac caccaagacc atcgccaagc gcctagaagc ctactaccga
      541 gcgtccatcc ccgtgatcgc ctactacgag acaaaaacac agctacacaa gataaatgca
      601 gagggaacac cagaggacgt ttttcttcaa ctctgcacag ctattgactc tattttcttg
      661 tgacagtaaa ggtggatac
//
