LOCUS       sequence-83812-e        1518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1518)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UTYC-c613
FEATURES             Location/Qualifiers
     source          1..1518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1435..1455
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1456..1485
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1486..1509
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaagaagga gatatactat gttgccaaag gacaagttga atccacccac acctagtatt
       61 tacttggaaa ataaacgtga tgctttcttt cctccattac atcaattttg tacaaatcca
      121 aaaaaccctg ttacagtaat acgtggcctt gctggagctc ttaaattaga tcttggactt
      181 ttctctacca aaactttggt agaagctaac aatgaacata tggtagaagt gaggacacag
      241 ttgctgcaac cagcagatga aaactgggat cccactggaa caaagaaaat ctggcgttgt
      301 gaaagcaata gatctcatac tacaattgcc aaatacgcac aataccaggc ttcctccttc
      361 caggaatcat tgagagaaga aaatgagaaa agaacacaac acaaagatca ttcagataac
      421 gaatccacat cttcagagaa ttctggaagg agaaggaaag gaccttttaa aaccataaaa
      481 tttgggacca acattgacct ctctgataac aaaaagtgga agttgcagtt acatgaactg
      541 actaaacttc ctgcttttgc gcgtgtggtg tcagcaggaa atcttctaac ccatgttggg
      601 cataccattc tgggcatgaa tacagtacaa ctgtatatga aagttccagg gagtcggaca
      661 ccaggtcacc aagaaaataa caacttctgc tctgttaaca taaatattgg tccaggagat
      721 tgtgaatggt ttgttgtacc tgaagattat tggggtgttc tgaatgactt ctgtgaaaaa
      781 aataatttga attttttaat gagttcttgg tggcccaacc ttgaagatct ttatgaagca
      841 aatgtccctg tgtatagatt tattcagcga cctggagatt tggtctggat aaatgcaggc
      901 actgtgcatt gggttcaagc tgttggctgg tgcaataaca ttgcctggaa tgttggtcca
      961 cttacagcct gccagtataa attggcagtg gaacggtatg aatggaacaa attgaaaagt
     1021 gtgaagtcac cagtacccat ggtgcatctt tcctggaata tggcacgaaa tatcaaagtc
     1081 tcagatccaa agctttttga aatgattaag tattgtcttt tgaaaattct gaagcaatat
     1141 cagacattga gagaagctct tgttgcagca ggaaaagagg ttatatggca tgggcggaca
     1201 aatgatgaac cagctcatta ctgtagcatt tgtgaggtgg aggtttttaa tctgcttttt
     1261 gtcactaatg aaagcaatac tcaaaaaacc tacatagtac attgccatga ttgtgcacga
     1321 aaaacaagca aaagtttgga aaattttgtg gtgctcgaac agtacaaaat ggaggaccta
     1381 atccaagttt atgatcaatt tacactagct ctttcattat catcctcatc tgcagagaac
     1441 ctctacttcc aatcgcacca tcatcaccat caccatcacc accatgatta caaggatgac
     1501 gacgataagt gaggatcc
//
