LOCUS       sequence-83824-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a hairpin specific for human AUF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Alspach E, Flanagan KC, Luo X, Ruhland MK, Huang H, Pazolli E, 
            Donlin MJ, Marsh T, Piwnica-Worms D, Monahan J, Novack DV, 
            McAllister SS, Stewart SA
  TITLE     p38MAPK plays a crucial role in stromal mediated tumorigenesis.
  JOURNAL   Cancer Discov. 2014 Mar 26.
  PUBMED    24670723
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2014 Mar 26."
COMMENT     Sequence Label: pLKO-shAUF1 hairpin 2
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..135
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    289..406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
       61 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      121 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      181 aggacgaaac accggagtaa gaacgaggag gatgaactcg agttcatcct cctcgttctt
      241 acttttttaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
      301 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      361 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      421 ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag
      481 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
      541 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
      601 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
      661 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgc
//
