LOCUS       sequence-83825-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a hairpin specific for human AUF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Alspach E, Flanagan KC, Luo X, Ruhland MK, Huang H, Pazolli E, 
            Donlin MJ, Marsh T, Piwnica-Worms D, Monahan J, Novack DV, 
            McAllister SS, Stewart SA
  TITLE     p38MAPK plays a crucial role in stromal mediated tumorigenesis.
  JOURNAL   Cancer Discov. 2014 Mar 26.
  PUBMED    24670723
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2014 Mar 26."
COMMENT     Sequence Label: pLKO-shAUF1 hairpin 1
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    296..413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc ggagagtggt tatgggaagg tatctcgaga taccttccca
      241 taaccactct tttttaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
      301 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      361 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      421 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
      481 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
      541 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
      601 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
      661 gaaggttcct tgcggttcgc ggcgtgccgg acgtga
//
