LOCUS       sequence-83827-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MDA5 with a mutation to motif III in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Bamming D, Horvath CM
  TITLE     Regulation of signal transduction by enzymatically inactive 
            antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.
  JOURNAL   J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 
            10.1074/jbc.M807365200. Epub 2009 Feb 11.
  PUBMED    19211564
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M807365200"; journalName: "J Biol Chem"; date: 
            "2009-04-10- 10"; volume: "284"; issue: "15"; pages: "9700-12"
COMMENT     Sequence Label: pEF-Bos-FLAG MDA5 MIII
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             55..78
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgaggattct ctagataagc caccatggat tataaggatg atgatgataa agatgattat
       61 aaggatgatg atgataaagg ttcgaatggg tattccacag acgagaattt ccgctatctc
      121 atctcgtgct tcagggccag ggtgaaaatg tacatccagg tggagcctgt gctggactac
      181 ctgacctttc tgcctgcaga ggtgaaggag cagattcaga ggacagtcgc cacctccggg
      241 aacatgcagg cagttgaact gctgctgagc accttggaga agggagtctg gcaccttggt
      301 tggactcggg aattcgtgga ggccctccgg agaaccggca gccctctggc cgcccgctac
      361 atgaaccctg agctcacgga cttgccctct ccatcgtttg agaacgctca tgatgaatat
      421 ctccaactgc tgaacctcct tcagcccact ctggtggaca agcttctagt tagagacgtc
      481 ttggataagt gcatggagga ggaactgttg acaattgaag acagaaaccg gattgctgct
      541 gcagaaaaca atggaaatga atcaggtgta agagagctac taaaaaggat tgtgcagaaa
      601 gaaaactggt tctctgcatt tctgaatgtt cttcgtcaaa caggaaacaa tgaacttgtc
      661 caagagttaa caggctctga ttgctcagaa agcaatgcag aga
//
