LOCUS       sequence-83829-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MDA5 with a mutation to motif I in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Bamming D, Horvath CM
  TITLE     Regulation of signal transduction by enzymatically inactive 
            antiviral RNA helicase proteins MDA5, RIG-I, and LGP2.
  JOURNAL   J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 
            10.1074/jbc.M807365200. Epub 2009 Feb 11.
  PUBMED    19211564
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M807365200"; journalName: "J Biol Chem"; date: 
            "2009-04-10- 10"; volume: "284"; issue: "15"; pages: "9700-12"
COMMENT     Sequence Label: pEF-Bos-FLAG MDA5 MI
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..41
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagataagc caccatggat tataaggatg atgatgataa aggtgattat aaggatgatg
       61 atgataaagg ttcgaatggg tattccacag acgagaattt ccgctatctc atctcgtgct
      121 tcagggccag ggtgaaaatg tacatccagg tggagcctgt gctggactac ctgacctttc
      181 tgcctgcaga ggtgaaggag cagattcaga ggacagtcgc cacctccggg aacatgcagg
      241 cagttgaact gctgctgagc accttggaga agggagtctg gcaccttggt tggactcggg
      301 aattcgtgga ggccctccgg agaaccggca gccctctggc cgcccgctac atgaaccctg
      361 agctcacgga cttgccctct ccatcgtttg agaacgctca tgatgaatat ctccaactgc
      421 tgaacctcct tcagcccact ctggtggaca agcttctagt tagagacgtc ttggataagt
      481 gcatggagga ggaactgttg acaattgaag acagaaaccg gattgctgct gcagaaaaca
      541 atggaaatga atcaggtgta agagagctac taaaaaggat tgtgcagaaa gaaaactggt
      601 tctctgcatt tctgaatgtt cttcgtcaaa caggaaacaa tgaacttgtc caagagttaa
      661 caggctctga ttgctcaga
//
