LOCUS       sequence-83841-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1855
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        154..258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             443..821
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(477..496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
       61 tgccagagtc gacagtatct tgttatccgc tcacaatgtc aatgttatcc gctcacattt
      121 atgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      361 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      421 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      481 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
      541 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      601 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      661 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      721 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      781 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      841 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggat
//
