LOCUS       sequence-83842-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, 
            PconNoHindM10:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1948
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        175..279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             464..842
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(498..517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacgcatg ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
       61 tgtgcaggtc cctgccagag tcgacctgtg tggaatccac cataaccgcc aagcataaaa
      121 atgtaaacaa cgacggtgct cgagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      181 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      241 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      301 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      361 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      421 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      481 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      541 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      601 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      661 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      721 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      781 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      841 ttgcgcaaac tattaactgg cgaactactt actctagctt
//
