LOCUS       sequence-83843-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st2):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1853
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        160..264
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(483..502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catgctaacc atgactaact ccttttttat catctctgga acacacacaa tccgtgtgca
       61 ggtccctgcc agagtcgaca gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      121 acatttatga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      181 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      241 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      301 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      361 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      421 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      481 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      541 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      601 gatggcatga cagtaagaga attatgcagt gctgcca
//
