LOCUS       sequence-83844-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1914
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        238..342
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(561..580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttctcctt tacgcatgcg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat
       61 tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt
      121 ctgaaaaaat tgcgcacgat acgtcgacct gtgtggaatc catcataacc gccaagcata
      181 aaaatgtaaa caacgacggt gctcgagacg tcaggtggca cttttcgggg aaatgtgcgc
      241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      361 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      421 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      481 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      541 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      601 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      661 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      721 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      781 accgcttttt tgcacaacat gggggatcat gtaactcgc
//
