LOCUS       sequence-83849-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:RBS(st7):gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1801
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(301..320)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca ttttttttcc tccttacccg ggagtatctt gttatccgct cacaatgtca
       61 atgttatccg ctcacattta tggatcagtc gacctgcagt tcgagacgtc ggaattgcca
      121 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
      181 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
      241 tttcgcatga ttgaacaagg tggattgcac gcaggttctc cggccgcttg ggtggagagg
      301 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
      361 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
      421 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
      481 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
      541 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
      601 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
      661 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
      721 gacgaagagc atcaggggct cgcgccaccc gaactgttcg ccaggctcaa ggcgcgcatg
      781 cccgacggcg aggacctcgt cgtgacccat ggcgatgcct gcttgcc
//
