LOCUS       sequence-83851-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st2):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1529
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        167..271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             456..834
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(490..509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttacgcat gctaaccatg actaactcct tttttatcat ctctggaaca cacacaatcc
       61 gtgtgcaggt ccctgccaga gtcgacagta tcttgttatc cgctcacaat gtcaatgtta
      121 tccgctcaca tttatgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      241 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      301 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      361 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      421 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      481 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      541 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      601 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      661 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      721 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      781 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
//
