LOCUS       sequence-83853-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1394
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        295..399
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(618..637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttacgcatgc tcgcggtacc tttaatcagg ttgttcaggt tagtgacata agtctgatgg
       61 tgcttgccgt agtgatactc gatggtttcc gcagaaatgt gcggtgccag agcatcttta
      121 gcatatggta gtgcaggtaa ttcgaatgac attgctactc tcctttatta ttaatttgca
      181 tacgtacaat agccttattg tgcgtatggt cgacagtatc ttgttatccg ctcacaatgt
      241 caatgttatc cgctcacatt tatgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      541 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      601 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      661 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      721 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      781 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      841 gctttt
//
