LOCUS       sequence-83855-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1405
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    222..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        296..400
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(619..638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacgcatg ctcgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg
       61 gtgcttgccg tagtgatact cgatggtttc cgcagaaatg tgcggtgcca gagcatcttt
      121 agcatatggt agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc
      181 atacgtacaa tagccttatt gtgcgtatgg tcgacagtat cttgttatcc gctcacaatg
      241 tcaatgttat ccgctcacat ttatgacgtc aggtggcact tttcggggaa atgtgcgcgg
      301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      361 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      421 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      481 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      541 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      601 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      661 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      721 agaaaagc
//
